Gene Wnk1 (Mouse)

Wnk1

Species: Mouse
Alias: Wnk1, 6430573H23Rik
External Links: MGI , Entrez Gene , Refseq cDNA , Refseq Protein , Phosphosite , iHOP
Annotation:

Classification

Group: Other
Family: WNK

Sequence

Name Sequence Type Origin Length Description Download
Wnk1.kin_dom Protein Kinase Domain Sugen global kinase HMM prediction 259 Kinase domain by global Sugen HMM Fasta, JSON
Wnk1.NA RNA Sugen 10568 None Fasta, JSON
Wnk1.AA Protein Sugen 2377 None Fasta, JSON

Protein domain

Protein domains of Wnk1.AA. The domains are annotated by HMM profiles from Pfam and SMART, as well as in-house data which includes HMMs of each individual kinase group, family and subfamily. Here is domain list in details, including sequence identity, significance and alignment. In particular, you can find can find the best hit kinase group/family/subfamily, which is helpful to understand the relationship between kinases.Kinase domain and best hit kinase group/family/subfamily are highlighted in red and blue. Visualized by pviz.

Usage: Zoom in selected region by dragging mouse and zoom out by double-clicking.

Show/hide domain list
Domain Protein name Domain Name Range Identity (%) Significance Score Profile Source Profile Range (length) Alignment
Kinase Wnk1.AA WNK 221-479 85 2.2e-234 764.71 In-house 1-261 (261) Show / Hide 
Range on Protein: 221-479
Range on HMM: 1-261/261
Sequence Identity: 85% (223 aa)

LKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMK
.|||.|.|||||||||.||||||.||||||||||.||.|.|||||||||||||.|||||||||||.|||...|||||||||||||||||||||||||.||
MKFDEEVGRGSFKTVYRGLDTETWVEVAWCELQDQKLSKNERQRFKEEAEMLKKLQHPNIVRFYDYWESVLCGKKCIVLVTELMTSGTLKTYLKRFKKMK

IKVLRSWCRQILKGLQFLHTRTP-PIIHRDLKCDNIFITGPTGSVKIGDLGLA-TLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATS
.||||||||||||||.|||||.| ||||||||||||||||.|||||||||||| |||..||||||||||||||||||||.||||||||||||||||||||
PKVLRSWCRQILKGLHFLHTRQPHPIIHRDLKCDNIFITGTTGSVKIGDLGLAVTLKNSSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATS

EYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFF
|||||||||||||||.||.|.||.||.|. .||||||||.|||.||.||.||..||||.||
EYPYSECQNAAQIYRKVTQGIKPNSFQKIQNPEVKEIIEKCIRHNKEERPSIQQLLNHDFF
Kinase Wnk1.AA S_TKc 221-479 24 7.1e-68 232.72 SMART 1-231 (231) Show / Hide 
Range on Protein: 221-479
Range on HMM: 1-231/231
Sequence Identity: 24% (68 aa)

LKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTS--GTLKTYLKRFKV
 ..  .||.|.| .|||. ...|  .||  ..          .... | ..||  .|||||..||....     ... .|.|..    |.|..|.|. . 
YEILRKIGKGAFGKVYKCRHKKTGRIVAIKIIK---------EHIRREIQILKK-HHPNIVKLYDVFQD-----DHLYMVMEYCDGDLGDLFDYIKKRGR

------MKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDE-SVDVYAFGMC
      .   . |....||...|...| .   ||||||| .||...     .||.| |||..      ....|||..||||.   .|.  ..|....| .
HGLRFPFPE-HARFYMYQICCALEYCHSH--GIIHRDLKPENILLDE---HIKICDFGLARQL-----TTFCGTPWYMAPEVL--GYGKCKCDWWSCGCI

MLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLN-------HAFF
. ||.. . |..   . .......              ||.|..|..| . . ..|... ..|.       | .|
LYEMLCGYPPFP---QMQMMFKKIG------------SPEAKDFIRKCLQKDPEKRPTA-EALQDEWDIKCHPWF
Kinase Wnk1.AA Pkinase 221-479 26 2.6e-61 214.8 Pfam 1-293 (293) Show / Hide 
Range on Protein: 221-479
Range on HMM: 1-293/293
Sequence Identity: 26% (81 aa)

LKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKE--EAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSG----TLKTYLK
...   ||.||| .|||. .  |  .||  ..  |.  ....|.     |....| |.||||||.||..|.    |. | .|.|..  |     |..|..
YHIGRKIGSGSFGCVYKCHHKGTGKIVAVKIIKKRHEKSWKDQTHRRLSEIQIMKRLNHPNIVRLYDWFED----KDHIYMVMEYCEGGLYVMDLFDYIS

--RFKVM--KIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSV----KIGDLGLAT---LKRASFAKSVIGTPEFMAPEM--YEEKYDE
  | ...    ........|||.||...| .   ||||||| .||.|   .| .    ||.| |||    . . | .... |||..||||     ..|. 
TWRHGCFYFSEWECKFYMYQILRGLEYCHSM--GIIHRDLKPENILID-NNGHIDACVKICDFGLAKQFDY-NNS-MTTFCGTPWYMAPEVIRGGQYYGP

SVDVYAFGMCMLEMATSEYPYSEC-----QNAAQIYRRVTSGVKPASFDKVAI-------------------PEVKEIIEGCIRQNKDERYSIKDLLNHA
.||....|  . ||.. . |....      .  |...|. ..  . ....                      .| |..|..|....  .|......|.| 
KVDMWSCGCILYEMLCGRPPFPGHCWHDNHDQMQMICRIMGPPLHFDWPWWCSISYFFFRHWHFHWTFHNWSEECKDFIKWCLCKDPKKRPTAEQILQHP

FF
.|
WF
Kinase Wnk1.AA FRWNK_Wnk1 480-571 80 8.8e-60 204.92 In-house 1-92 (92) Show / Hide 
Range on Protein: 480-571
Range on HMM: 1-92/92
Sequence Identity: 80% (74 aa)

QEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQ
||.||||||||||||||| ||||||..|| ||||||||||||||||||||.||||.|||||||||..|| |.|..||.|.|||.|||.|||.
QEDTGVRVELAEEDDGEKSAIKLWLWMEDPKKLKGKYKDNEAIEFSFDLEKDVPEEVAQEMVESGFFCEADYKIVAKSIRDRVALIKWKRER
Kinase Wnk1.AA STE 221-479 20 3.4e-59 202.99 In-house 1-359 (359) Show / Hide 
Range on Protein: 221-479
Range on HMM: 1-359/359
Sequence Identity: 20% (76 aa)

LKFDIEIGRGSFKTVYKGLDTETT-----------------VEVAWCELQD-------------RKLTKSERQRFKEEAEMLKGLQ--HPNIVRFYDSWE
...  .||.|.| ||||... ...                 . .   .|..             ..  ..|.|....| ...| ..  |||||..|.. .
WEGIEKIGKGAFGTVYKAMNHKNCNIHKTGEVENNGETGEIMAIKQMNLENCNNKDENQENQELENKNHEEMQQILKEIKIMKKCNHMHPNIVKYYGCYF

ST---VKGKKCIVLVTELMTSGTLKTYLK-RFKVMKI-------------KVLRSWCRQILKG-LQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLG
      | .  . .. |.|  |.|   .| .... |              ....  ||||||| |..||     |||||.|..||..      ||. | |
KEGHNHKNDDELWICMEYMDGGSLDDIIKHKYGCGKPLNHNRQHQHRFPESQIAYICRQILKGLLYYLHSHH--IIHRDIKPANILL------VKLCDFG

LATLKRAS------------------FAKSVIGTPEFMAPEMYEE-------KYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSG-------
...    |                    ....||| .||||. .        .||...|.. .| ...|||| ..|... ..   ...  ...       
VCGQLTDSMANIQSNNIIDCIETMQQKRNTFVGTP-WMAPEVIQQNGQGRDKGYDTKCDIWSLGCTIIEMATGKPPWHDMHPMRAMFMIPFNYHMQQQFE

------VKPASFDKVAI-------------------PEVKEIIEGCIRQNKDERYSIKDLLN--HAFF
       .| ..                        || |. |. | . . ..| . ..||.  | |.
QLCIVNGPPPPLPDHLSQNEMISYGGSGTTYPHKWSPEFKDFINKCLQKDPNKRPTASQLLKSEHPFI
Kinase Wnk1.AA CAMK 226-479 17 2.2e-56 179.82 In-house 1-425 (425) Show / Hide 
Range on Protein: 226-479
Range on HMM: 1-425/425
Sequence Identity: 17% (74 aa)

EIGRGSFKTVYKGLDTETT------------VEVAWCELQDRKLTKSE--------------------------RQRFKEEAEMLKGLQ-------HPNI
..||||| .|... ...||            ..||   .  .||....                          |.... | |..| ||       ||||
TLGRGSFGKVKLCIHKNTTGKERVHHIYTNGQKVAIKIIDKKKLKDKQNQDYSNCYKIDKSKCKHRYCWRRPKDRKNVRREIEIMKKLQGIGNKLDHPNI

VRFYDSWES-----------TVKG-----KKCIVLVT---ELMTSG----TLKTYLKRF---------------KVMKIKVLRSWCRQILKGLQFLHTRT
...|. .|.           | .      .|....|.   |... |     |  |...                . .. .  | . |||. .....| . 
IKLYEVYEDPHYFKNTNDYET-SKQVFTKDKHMYMVMEIMEYCSGGELFDDLFDYIVEHGCYGEEFDQFDIVERGRFTEREARRYFRQICSAVHYCHSHN

PPIIHRDLKCDNIFITGPT----------------GSVKIGDLGLATLKRAS-------------------------FAKSVIGTPEFMA-PEMYEEK--
  |.|||||..|| ..                   . .|| | |.|. .. .                           | . ||||.|| ||. . |  
--IVHRDLKPENILLDDNNDDSDPVEHDEIFDWNNNNIKIIDFGFANKFDPGENNFMCSDYKGGEMHNQWTTPTEGQKMKTFCGTPEYMAAPEVLNGKGH

-------------YDESVDVYAFGMCMLEMATSEYPYSEC------------QNAAQIYRRVTSGVKPASFDKVAI----------------------PE
             |....|. . |  .  | . ..|. .             .|  ........|      ... .                      .|
CNMQNATDEEKPYYGPKCDMWSCGVILYIMLCGYPPFYGDCDNEICEDEWIGMNDYELFEKIMKGKYDFPPPYFSPISCNYGPEWWDDISDEFHHFVSQE

VKEIIEGCIRQNKDERYSIKDLLNHAFF
.|  | .. . . ..|..|.. ||| ..
CKDLIRKMLQVDPEKRMTIEQCLNHPWM
Kinase Wnk1.AA Ciliate-E2 221-479 18 1.6e-50 171.66 In-house 1-365 (365) Show / Hide 
Range on Protein: 221-479
Range on HMM: 1-365/365
Sequence Identity: 18% (74 aa)

LKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSE---------------------RQRFKEEAEM---------------LKGLQHPNIVRFY
 ||   ||.|.| .|||. . ..  ..|    . .|. ..                      .| ..|. .|               |. |.||||...|
YKFIKKIGQGNFGSVYKCKVWKVQKQFAIKIID-QKQ-QRQQMQEKVKIKIYAMKIMKKNQIEQIKQEIHIMREINCFYKKINEKNILEQLNHPNIIKMY

DSWESTVKGKKCIVLVTELMTSGTLKTYLK-----RFKV------MKIKVLRSWCRQILK---------------GLQFLHTRTPPIIHRDLKCDNIFIT
.....  | ...| ||.|.   |.|. |.|     |..       ...|  .   .|||.               .|..|| . . |||||.| .||.. 
ECFQE--KDDNNIYLVMEYCQGGDLFDYIKNNKKKRNHEIEKDKYFTEKEIKYIMKQILQGFKTKAEFYIACIILALEYLHSKNN-IIHRDIKPENILF-

GPTGSVKIGDLGLAT-----------------LKRAS-----------------FAKSVIGTPEFMAPEMYEEK----------YDESVDVYAFGMCMLE
     .|| |.||..                 ..  .                  .....|||..||||... |          ||...|....|... |
-----IKIIDFGLSKKYDDDNQNRTHKQQCKWYMF-KNDTSRTPGYMDQIIQQNMFNTICGTPGYMAPEILNGKKYDNQQKQKGYDYKCDIWSLGVILYE

MATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAI----------------------------PEVKEIIEG----CIRQNKDERYS-IKDLLNHAFF
| . ..|.                     ...                               .|.| .|      ..  . ..|.. ....||| .|
MLFGKPPF---------------------QQQIKQCKIDFPCPEWQKDTQCIQCKWKSWKKWSQECKDLIKKFGKRMLQKDPEKRITGWEQILNHPWF
Kinase Wnk1.AA TKL 227-477 19 1.4e-48 167.76 In-house 1-364 (364) Show / Hide 
Range on Protein: 227-477
Range on HMM: 1-364/364
Sequence Identity: 19% (73 aa)

IGRGSFKTVYKGLDTETT--------VEVAWCELQDRKLTKSER-------------------------QRFKEEAEMLKGLQHPNIVRFYDSWESTVK-
||.|.| .||||    ..         .||   .  .   ....                         ....||. |.. |.|||||.|. .  .  . 
IGSGGFGEVYKGKWRGWGGKCPSDTGKDVAVKKFKSHNDDEFTKNSQENNDNNNYSDMQLNDPEASWFRFFEEEEIMMMSRLRHPNIVQFMGYCCCPPCG

--------GKKCIVLVTELMTSGT-LKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRT------PPIIHRDLKCDNIFITG-------------PTGSV
        ... . .|.|..  |. | ...       . .. .|...| .|...||...      ||||||||| .||..                .. .
CVCMHPVGPWGQLCIVMEYCPGGSLLHYLH-------WHQRCRWALDIARGMNYLHSMNPKWCTKPPIIHRDLKSKNILVDENWTNVSNYMYNPNADWCC

KIGDLGLATLKRASF---------------------AKSVIGTPEFMAPEMYE-----------------EKYDESVDVYAFGMCMLEMATSEYPYSECQ
||.|.||.. .  |.                       ...||| .||||. .                 ..|.|..|||.||... |..| . ||..  
KICDFGLSRFMSQSGNMNDTMTTMMESAEHQNNRKTTMTQCGTPRWMAPEVLRGQMNYTEKVGIDEFCKGFEYSEKCDVYSFGIVLWEILTRCRPYYDYP

NAAQI---------YRRVTSGVKPASF-----------DKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHA
.  .|         . ..  | .|               . . |..|.... |  .. ..| | ...| . 
YMPMIFQDPFEEMFMMVCDKGLRPPIPPIWQNHHKMCPCPDCPPSMKKLMQDCWDHDPEKRPSFQEILKRL
Kinase Wnk1.AA ULK 221-479 22 4.6e-44 151.73 In-house 1-329 (329) Show / Hide 
Range on Protein: 221-479
Range on HMM: 1-329/329
Sequence Identity: 22% (76 aa)

LKFDIEIGRGSFKTVYKGLDTETT----VEVAWCELQDRKLTKSERQR--------FKEEAEMLKGL--------QHPNIVRFYDSWESTVKGKKCIVLV
   . ..| ||| .|||| . .|     . ||   ..  |. ... ..        .| | . .| |        .|||||..||  |..    ..| ..
YCLNHCLGKGSFGKVYKGRNKKTKDYVNEKVACKMIDKQKMNQKNNEQEGQYMWQLLKNEIKIMKKLKSPNRHKNHHPNIVKLYDWFETSNH--NNIYII

TELMTSGTLKTYLKRFKVMKIKV----------LRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGS-----------VKIGDLGLATLKRAS-
.|.   |.|..|.|. | .  ..           .. ..||| |...|| .   |||||.| .|| |. ....           .||.| |.|     . 
MEYCNGGDLEQYIKKNKKLPEDTEEKKRLSEQEAIQFFKQILNGFKELHKH--NIIHRDIKPQNILIHNGQPKTDSKKVHPNGVLKIADFGFAKHVDNDN

----FAKSVI------GTPEFMAPEMYEEK-----YDES-VDVYAFGMCMLEMATSEYPYSECQNAAQI----YRRVTSG--------VKPASFD-----
     ...        ||| .|||.  ...     |... .|.. .|. . || . ..|.... .  |.    .. .  .         .|  |.     
MEQQMMMTCVNEKRLCGTPLYMAPQILQGQGEQKPYYDYKCDIWSLGCIFYEMLYGKPPFYAW-FNDQYLQIIQKLINNPPPWPQQIKKQPLQFPKDGND

KVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFF
..  ||.|..| ...  . ..| |. .|.|| .|
PQISPECKDLIRRMLQYDEKDRISWEELFNHPWF
Kinase Wnk1.AA NRBP 226-480 33 9e-43 148.15 In-house 1-261 (261) Show / Hide 
Range on Protein: 226-480
Range on HMM: 1-261/261
Sequence Identity: 33% (88 aa)

EIGRGSFKTVYKGLDTETTVEVAWCELQ--DRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFK----VM
       ...|  .|||. ||| | |.|  .||  |..  . ..  . |  |.|.|||.|   |  | . |  .. .|| | || .| .||. |     |
QRDVPGIDCAYLAMDTEEGVEVVWNEVQFSERKNFKAQEEKIRQVFDNLIQLDHQNIVKFHKYWTDTKEEKPRVIFITEYMSSGSMKQFLKKTKKNHKNM

KIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRA-SFAKSVIGTPEFMAPEMY---EEKYDESVDVYAFGMCMLEM
..|  . || |||  | .||.  |||||  | || |||    | .|||     ....    ..       |.|||      ..    ||.|.|||| |||
NEKAWKRWCTQILSALSYLHSCDPPIIHGNLTCDTIFIQH-NGLIKIGCVWPDAIHHHVRTCREEMRNMHFFAPEYGILEVTDLTTAVDIYSFGMCALEM

ATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQ
|  |   | |||.   |  ||  .   .   .  |  ...|. |....  .| | ..|| |    
AVLEI-QSGCQNGESEY--VTEEAIQRAIHSLEDPMQRDFIRKCLQKDPARRPSARELLFHQILF
Kinase Wnk1.AA STE11 227-479 25 8.3e-42 145.22 In-house 7-303 (303) Show / Hide 
Range on Protein: 227-479
Range on HMM: 7-303/303
Sequence Identity: 25% (77 aa)

IGRGSFKTVYKGLDTETTVEVA--WCELQDRK----LTKSERQR------FKEEAEMLKGLQHPNIVRFYDSWESTVKG-KKCIVLVTELMTSGTLKTYL
||.|.| ||||.... | ...|    |.|. .     .. | |       ..|| ...|.|.|.||||.|..  .  .. ....    | .  |.|.  |
IGKGAFGTVYKCMNVDTGELMAVKQIEIQNNNQMKEDQEKEYQSHKMHKAIQEEIKLMKHLNHKNIVRYYGCCLH--DNQNDHVNIFMEYCPGGSLSSML

K-RFKV--MKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRAS------------FAKSVIGTPE--------FM
. ..    .  .| |   .|||.|||.||     |||||.| .||..    ...|..| | |.   ..            . ||. |||         .|
RHKWGPMKFDEMVIRKYTKQILEGLQYLHSHG--IIHRDIKGANILLDSDGNVCKLADFGCAKRLAWICTCTHNMNMTQNHCKSMTGTPYNSMMGTPMWM

APEMYE-EK---YDESVDVYAFGMCMLEMATSEYPYSECQN--AAQIYRRVTS-GVKPA---SFDK--VAIPEVKEIIEGCIRQNKDERYSIKDLLNHAF
|||..  |.   |. ..|. ..| ...||||   |......  .|.... ... .  |    ..    .. .| ....  |  ... .|..  .||.| |
APEVIKQEGRDGYGRKADIWSLGCTVIEMATGKPPWHNYDHNHMAAMFKIGMGHKPHPPIPEHMSPISWISEECRDFLRHCFEHDPKKRPTANQLLQHPF

F
.
I
Kinase Wnk1.AA ULK 221-479 22 1.3e-41 144.55 In-house 1-324 (324) Show / Hide 
Range on Protein: 221-479
Range on HMM: 1-324/324
Sequence Identity: 22% (76 aa)

LKFDIEIGRGSFK-TVYKGLDTETT---VEVAWCELQDRKLTKSERQ--------RFKEEAEMLKGLQ---HPN--IVRFYD-SWESTVKGKKCIVLVTE
   . ..| |||  .|||| . .|    . ||    .  |. |.. .         .| | . .| |    |||  ||..|| ...|.    . | ...|
YCLNHCLGKGSFGVKVYKGRNKKTNTQNQYVACKMIDKKKMNKKKNEQEGQYMWQLLKNEIQIMKKLKELKHPNHHIVKLYDWFETSNH---NNIYIIME

LMTSGTLKTYLKRFKVMKIKV----------LRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGS-----------VKIGDLGLATL-----KR
.   |.|..|.|. |  .  .           .. ..||. |...|| .   |||||.| .|| |. ....           .||.| |.|       . 
YCNGGDLEQYIKKNKKLPE-TEEKKRLSEQEAIQFFKQICNGFKELHKH--NIIHRDIKPQNILIHNGQPKTDSKKVHPNGVLKIADFGFAKHVDNNNME

ASFAKSVI------GTPEFMAPEMYEEK-----YDES-VDVYAFGMCMLEMATSEYPYSECQNAAQI------------------YRRVTSGVKPASFDK
.   ..        ||| .|||.  ...     |... .|.. .|. . || . .||.... .  |.                  .. .    .|   |.
QQMCQTCVNEKRIKGTPLYMAPQILQGQGEQKPYYDYKCDIWSLGCIFYEMLYGKYPFFSW-FNDQYLYIQKLINNGKEQQIYIKNQPIQFPKPP--KDP

VAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFF
.  ||.|..| ...  . ..| |. ...|| .|
KISPECKDLIRRMLQYDEKDRISWEEIFNHPWF
Kinase Wnk1.AA NEK 223-417 29 1.3e-36 126.58 In-house 1-219 (286) Show / Hide 
Range on Protein: 223-417
Range on HMM: 1-219/286
Sequence Identity: 29% (65 aa)

FDI--EIGRGSFKTVYKGLDTETTVE-----VAWCELQDRKLTKSERQRFK-EEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMT-SGTLKTY-
..|  .||.||| .|||  ....  .     ..| . .  ...  |.|    .|.  || |.|||||..|||..   ... |.  | |     |.|    
YEIIKKIGQGSFGKVYKVRRKSDGKHLVEKLYVWKKINMGNMSEKEKQNAQNNEVRILKQLKHPNIVKYYDSFIE--EQNNCLCIVMEYCEGGGDLYQKI

--LKRFK----VMKIKVLRSWCRQILKGLQFLHTR-TP--PIIHRDLKCDNIFITG-PTGS--VKIGDLGLATLKRAS--FAKS-VIGTPEFMAPEMY-E
  .|. |     .  ..   |. |....|...| |  |   |.|||.|. |||.|   ...  |||||.|.. .   .  .|.. ..||| .|.||.  .
KSIKKQKQKGKYFEEEQIWDWFIQMCLALKYMHDRKGPQRKILHRDIKPQNIFLTKNNNDYHNVKIGDFGISKVLNHTTDMAYTTQVGTPYYMSPEVCQN

EKYDESVDVYAFGMCMLEMAT
..|.. .|. ..| .  ||.|
KPYNNKSDIWSLGCVLYEMCT
Kinase Wnk1.AA TyrKc 221-479 24 4e-22 80.73 SMART 1-280 (280) Show / Hide 
Range on Protein: 221-479
Range on HMM: 1-280/280
Sequence Identity: 24% (72 aa)

LKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGL--QHPNIVRFYDSWESTVKGK-------KCIVLVTELMTSGTLKT
.... ..|.| | .||||  .   ..||   |.. .     |. | .||.... |  .||||||.|      .  .       . . .|.|.|  | || 
ITLGKKLGEGAFGEVYKGTWK---IQVAVKMLKEDA-----REDFMREARIMCKLGGHHPNIVRLY----GVCTQQGRNFMYIEPLMIVMEYMPHGDLKD

YLKRFKV-MKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRAS----FAKSVIGT-----PEF------MAPEMY
||.. .   . | | |.| || .| ..|| .  ..||||| . |. .. .   |||.| ||.           .|... .       .      ||||  
YLRKHRPKLCMKDLLSFCWQIACGMEYLHSK--NCIHRDLAARNCLVGENH-TVKISDFGLSRDIYDDDQQGESKDYYRKKEHKGGKTMLPIRWMAPECI

EEK-YDESVDVYAFGMCMLEMATSEYPYSEC-----QNAAQIYRRVTSGVKPASFDKV-------AIPEVKEIIEGCIRQNKDERYSIKDLLNHAFF
 .. . .  ||..|| || |. |   .  .       ........| .|  .   . .       . .|. .|.. | . . ..| . ...  | .|
NYGKFTTKSDVWSFGVCMWEIFTYGQQPYPGMIQQMMSNQEVIEYVKQGYRMPQPPNDYPMSFVCCPDEMYQIMKQCWHEDPEDRPTFSEI--HECF
Kinase Wnk1.AA OSR1_C 500-537 65 2e-20 77.76 Pfam 1-38 (38) Show / Hide 
Range on Protein: 500-537
Range on HMM: 1-38/38
Sequence Identity: 65% (25 aa)

IKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVA
|.||||..| ||.|||.|.||||.|.||.|.|.||.||
INLWLRMRDPKKRKGKHKENEAIQFEFDIEKDTPEEVA
Kinase Wnk1.AA Pkinase_Tyr 299-372 37 2.3e-12 42.51 Pfam 84-158 (270) Show / Hide 
Range on Protein: 299-372
Range on HMM: 84-158/270
Sequence Identity: 37% (29 aa)

LVTELMTSGTLKTYLKR----FKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLA
.|.|.|  | |..||..     .  ..| | ..|.|| ||...|. .   .||||| . |. .| .   |||.| |||
IVMEYMPHGDLLDYLRKHREDKQPLTMKDLLQMCWQIAKGMEYLESKN--CIHRDLAARNCLVTENN-VVKICDFGLA
Kinase Wnk1.AA Pkinase_Tyr 221-284 35 5.4e-11 37.87 Pfam 1-70 (270) Show / Hide 
Range on Protein: 221-284
Range on HMM: 1-70/270
Sequence Identity: 35% (25 aa)

LKFDIEIGRGSFKTVYKGLDT----ETTVEVAWCELQDRKLTKSE--RQRFKEEAEMLKGL-QHPNIVRFY
..|. ..|.|.| .||||  .    .|...||   | . . ....  |. | .||...| | .||||||.|
ITFGKKLGEGAFGEVYKGTWKGDPDNTKIPVAVKMLKE-GAMHEQNCREDFMREAKIMKQLGHHPNIVRLY
Kinase Wnk1.AA FRWNK_Wnk2.hmm 524-569 30 3.3e-07 21.7 In-house 18-63 (63) Show / Hide 
Range on Protein: 524-569
Range on HMM: 18-63/63
Sequence Identity: 30% (14 aa)

FSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKR
| |||. | ||..|| |||. .. | . ..  . ..| .   . . 
FKFDLDGDNPEEIAQYMVENDFILESEREKFIEQMRDIIQKAEEMM
Kinase Wnk1.AA NEK 450-479 16 0.000453 10.68 In-house 257-286 (286) Show / Hide 
Range on Protein: 450-479
Range on HMM: 257-286/286
Sequence Identity: 16% (5 aa)

PEVKEIIEGCIRQNKDERYSIKDLLNHAFF
.. ...|. ... . ..|.|....|.. | 
QDLQNLISQMLQKDPEQRPSCNQILEMPFI
Kinase Wnk1.AA Pkinase_Tyr 390-438 32 0.025509 8.48 Pfam 181-232 (270) Show / Hide 
Range on Protein: 390-438
Range on HMM: 181-232/270
Sequence Identity: 32% (17 aa)

MAPE-MYEEKYDESVDVYAFGMCMLEMAT-SEYP-YSECQNAAQIYRRV-TSG
|||| . . |. .  ||..|| .| |. |  | | | ..   ...|...  .|
MAPECINYGKFTTKSDVWSFGVLMWEIFTYGEQPTYPGM-SNEEVYEYIYEDG
FRWNK_Wnk2.hmm Wnk1.AA FRWNK_Wnk2.hmm 1125-1187 76 2.1e-43 143.57 In-house 1-63 (63) Show / Hide 
Range on Protein: 1125-1187
Range on HMM: 1-63/63
Sequence Identity: 76% (48 aa)

DRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVAQVREIIEKADEML
||.|||||||||.||||||||||||||||||..||.|||||. |||.|..|.|.||.||.||.
DRMVECQLETHNHKMVTFKFDLDGDNPEEIAQYMVENDFILESEREKFIEQMRDIIQKAEEMM
FRWNK_Wnk1 Wnk1.AA FRWNK_Wnk1 1142-1189 27 0.000761 11.22 In-house 45-92 (92) Show / Hide 
Range on Protein: 1142-1189
Range on HMM: 45-92/92
Sequence Identity: 27% (13 aa)

FKFDLDGDNPEEIATIMVNNDFILAIERESFVAQVREIIEKADEMLSE
| |||. | |||.|  ||.. |..  . .     .|. .    .   .
FSFDLEKDVPEEVAQEMVESGFFCEADYKIVAKSIRDRVALIKWKRER
OSR1_C Wnk1.AA OSR1_C 1142-1155 57 0.124658 9.68 Pfam 25-38 (38) Show / Hide 
Range on Protein: 1142-1155
Range on HMM: 25-38/38
Sequence Identity: 57% (8 aa)

FKFDLDGDNPEEIA
|.||.  | |||.|
FEFDIEKDTPEEVA
Wnk.CR4 Wnk1.AA Wnk.CR4 2052-2127 61 2e-45 157.22 In-house 1-80 (80) Show / Hide 
Range on Protein: 2052-2127
Range on HMM: 1-80/80
Sequence Identity: 61% (49 aa)

SYMSSDNESDIEDEDLRLELRRLREKHLKEIQDLQSRQKHEIESLYTKLG-KVPPAVIIPP---AAPLSGRRRRPTKSKG
|||||||.|..||||.. ||.||||||.||||.||..||.|||.|||..| ..||....|.   .||.|||||||.|.|.
SYMSSDNDSEFEDEDMWKELQRLREKHMKEIQELQAQQKKEIEELYTRMGKPPPPGIVSPHAMLTAPMSGRRRRPSKHKS